Scientific publications

Migrating cells often encounter a wide variety of topographic features—including the presence of obstacles—when navigating through crowded biological environments. Unraveling the impact of topography and crowding on the dynamics of cells is key to better understand many essential physiological processes such as the immune response. We study the impact of geometrical cues on ameboid migration of HL-60 cells differentiated into neutrophils. A microfluidic device is designed to track the cells in confining geometries between two parallel plates with distance h, in which identical micropillars are arranged in regular pillar forests with pillar spacing e. We observe that the cells are temporarily captured near pillars, with a mean contact time that is independent of h and e. By decreasing the vertical confinement h, we find that the cell velocity is not affected, while the persistence reduces; thus, cells are able to preserve their velocity when highly squeezed but lose the ability to control their direction of motion. At a given h, we show that by decreasing the pillar spacing e in the weak lateral confinement regime, the mean escape time of cells from effective local traps between neighboring pillars grows. This effect, together with the increase of cell-pillar contact frequency, leads to the reduction of diffusion constant D. By disentangling the contributions of these two effects on D in numerical simulations, we verify that the impact of cell-pillar contacts on cell diffusivity is more pronounced at smaller pillar spacing.

Thin, elastic sheets are well known to adapt to rough counterfaces, whereby adhesive interactions and pull-off stresses σ_p can be significant, yet no generally applicable, quantitative guideline has been suggested hitherto as to when a sheet should be considered thin enough to be sticky. Using computer simulations, we find that the dependence of σ_p on surface energy γ has a high and a low-pull-off-stress regime. For randomly rough surfaces, we locate the dividing line at the point, where γ is approximately half the elastic energy per unit area needed to make conformal contact, which is the same ratio as for semi-infinite elastic solids. This rule of thumb also applies to a certain degree for single-wavelength roughness, in which case the transition from low to high stickiness occurs when at the moment of maximum tension contact is not only broken at the height maxima but also at the saddle points.

We study interacting active Brownian particles (ABPs) with a space-dependent swim velocity via simulation and theory. We find that, although an equation of state exists, a mechanical equilibrium does not apply to ABPs in activity landscapes. The pressure imbalance originates in the flux of polar order and the gradient of swim velocity across the interface between regions of different activity. An active–passive patch system is mainly controlled by the smallest global density for which the passive patch can be close packed. Below this density a critical point does not exist and the system splits continuously into a dense passive and a dilute active phase with increasing activity. Above this density and for sufficiently high activity the active phase may start to phase separate into a gas and a liquid phase caused by the same mechanism as motility-induced phase separation of ABPs with a homogeneous swim velocity.

Visualizing interactions between cells and the extracellular matrix (ECM) mesh is important to understand cell behavior and regulatory mechanisms by the extracellular environment. However, long term visualization of three-dimensional (3D) matrix structures remains challenging mainly due to photobleaching or blind spots perpendicular to the imaging plane. Here, we combine label-free light-sheet scattering microcopy (LSSM) and fluorescence microscopy to solve these problems. We verified that LSSM can reliably visualize structures of collagen matrices from different origin including bovine, human and rat tail. The quality and intensity of collagen structure images acquired by LSSM did not decline with time. LSSM offers abundant wavelength choice to visualize matrix structures, maximizing combination possibilities with fluorescently-labelled cells, allowing visualizing of long-term ECM-cell interactions in 3D. Interestingly, we observed ultrathin thread-like structures between cells and matrix using LSSM, which were not observed by normal fluorescence microscopy. Transient local alignment of matrix by cell-applied forces can be observed. In summary, LSSM provides a powerful and robust approach to investigate the complex interplay between cells and ECM.

Mussels use byssal threads to secure themselves to rocks and as shock absorbers during cyclic loading from wave motion. Byssal threads combine high strength and toughness with extensibility of nearly 200%. Researchers attribute tensile properties of byssal threads to their elaborate multi-domain collagenous protein cores. Because the elastic properties have been previously scrutinized, we instead examined byssal thread viscoelastic behaviour, which is essential for withstanding cyclic loading. By targeting protein domains in the collagenous core via chemical treatments, stress relaxation experiments provided insights on domain contributions and were coupled with in situ small-angle X-ray scattering to investigate relaxation-specific molecular reorganizations. Results show that when silk-like domains in the core were disrupted, the stress relaxation of the threads decreased by nearly 50% and lateral molecular spacing also decreased, suggesting that these domains are essential for energy dissipation and assume a compressed molecular rearrangement when disrupted. A generalized Maxwell model was developed to describe the stress relaxation response. The model predicts that maximal damping (energy dissipation) occurs at around 0.1 Hz which closely resembles the wave frequency along the California coast and implies that these materials may be well adapted to the cyclic loading of the ambient conditions.

Spaceflight is facing a sustainability problem in Earth orbit, where about 90% of all man-made trackable objects are without functional use. Existing research activities on active debris removal are technologically complex and costly, which are potential reasons why no missions were carried out so far. Micropatterned dry adhesives inspired from climbing animals, such as geckos and beetles, have been proposed as a radically new docking and capture approach for non-cooperative targets. Their successful implementation is expected to significantly reduce the technical complexity and the overall mission cost. In this article, recent developments of micropatterned dry adhesives are reviewed with a focus on space applications and their use for active debris removal. The problem and solutions for active debris removal are analyzed and open issues that need to be addressed by future work are discussed.

We analyse crack growth in viscoelastic material by use of a correspondence principle that allows elasticity solutions for traction boundary value problems in plane strain to be converted to viscoelasticity solutions. We consider an edge cracked strip in tension and assess 2 limiting cases of the geometry. In one case, we allow the component to become a geometry that is an infinite body with a semi-infinite crack, or a large body with a long crack. In the other case, we consider a component of finite width with a remaining ligament that is very small compared to the width of the strip. In these geometries we compute the work done by the applied load per unit area of crack growth and a measure that we consider to represent the dissipation per unit area of crack growth. We do so for a standard viscoelastic material with a single retardation time and for a Maxwell material with a single viscous element. In the latter case, our computation of the dissipation per unit area of crack growth is definite. We consider various aspects of the behaviour of the work done per unit area of viscoelastic crack propagation and the dissipation per unit area of crack advance. These include the extent to which these parameters depend on the rate of crack propagation, the extent to which these parameters are independent or dependant on component geometry, and the extent to which these parameters exhibit transient behaviour during crack growth at a steady rate under a constant applied stress intensity factor. A motivation is the common insight that a parameter is probably more useful as a measure of material behaviour if it is relatively insensitive to component geometry and if, during steady state response in terms of crack growth rate and applied stress intensity factor, the parameter also exhibits a steady state. We find outcomes that vary quite considerably depending on the case that we consider. We provide our results for the reader to use when considering the various models that have been proposed in the literature for viscoelastic crack propagation. We observe, however, that crack propagation models based on a rupture process zone interacting with material viscoelasticity are much simpler to implement in bodies with finite geometry than those based on quantifying the dissipation per unit area of crack growth. In this regard, we conclude that viscoelastic crack growth models that are based on quantifying the dissipation per unit area of crack growth are, by themselves, not a promising concept as we see no obvious way to extend them to provide unique results in components having a finite geometry. We further conclude that a reliable viscoelastic crack growth model should include a crack tip rupture process zone at the crack tip.

Adhesives for interaction with human skin and tissues are needed for multiple applications. Micropatterned dry adhesives are potential candidates, allowing for a conformal contact and glue-free adhesion based on van der Waals interactions. In this study, we investigate the superior adhesion of film-terminated fibrillar microstructures (fibril diameter, 60 μm; aspect ratio, 3) in contact with surfaces of skin-like roughness (Rz 50 μm). Adhesion decays only moderately with increasing roughness, in contrast to unstructured samples. Sinusoidal model surfaces adhere when their wavelengths exceed about four fibril diameters. The film-terminated microstructure exhibits a saturation of the compressive force during application, implying a pressure safety regime protecting delicate counter surfaces. Applications of this novel adhesive concept are foreseen in the fields of wearable electronics and wound dressing.

Fibrillar dry adhesives have shown great potential in many applications thanks to their tunable adhesion, notably for pick-and-place handling of fragile objects. However, controlling and monitoring alignment with the target objects is mandatory to enable reliable handling. In this paper, we present an in-line monitoring system that allows optical analysis of an array of individual fibrils (with a contact radius of 350 µm) in contact with a smooth glass substrate, followed by the prediction of their adhesion performance. Images recorded at maximum compressive preload represent characteristic contact signatures that were used to extract visual features. These features, in turn, were used to create a linear model and to train different linear and non-linear regression models for predicting adhesion force depending on the misalignment angle. Support vector regression and boosted tree models exhibited highest accuracies and outperformed an analytical model reported in literature. Overall, this new approach enables predictions in gripping objects by contact observations in near real-time, which likely improves the reliability of handling operations.

Reversible and switchable adhesion of elastomeric microstructures has attracted significant interest in the development of grippers for object manipulation. Their applications, however, have often been limited to dry conditions and adhesion of such deformable microfibrils in the fluid environment is less understood. In the present study, we performed adhesion tests in silicone oil using single cylindrical microfibrils of a flat-punch shape with a radius of 80 µm. Stiff fibrils were created using three-dimensional printing of an elastomeric resin with an elastic modulus of 500 MPa, and soft fibrils, with a modulus of 3.3 MPa, were moulded in polyurethane. Our results suggest that adhesion is dominated by hydrodynamic forces, which can be maximized by stiff materials and high retraction velocities, in line with theoretical predictions. The maximum pull-off stress of stiff cylindrical fibrils is 0.6 MPa, limited by cavitation and viscous fingering, occurring at retraction velocities greater than 2 µm s−1. Next, we add a mushroom cap to the microfibrils, which, in the case of the softer material, deforms upon retraction and leads to a transition to a hydrostatic suction regime with higher pull-off stresses ranging from 0.7 to 0.9 MPa. The effects of elastic modulus, fibril size and viscosity for underwater applications are illustrated in a mechanism map to provide guidance for design optimization.