Dahmen, Tim | Trampert, Patrick | de Jonge, Niels | Slusallek, Philipp
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Three-dimensional (3D) scanning transmission electron microscopy (STEM) has become one of the primary tools for analytical characterization in materials science and also finds increasing use in the life sciences. A number of different recording schemes exist for the acquisition of 3D data using STEM, each capturing different spatial frequencies and, thus, different information about the shape of a specimen. In this article, we present and compare different sampling approaches based on images with both large and small depth of field. We highlight the latest contribution to 3D data acquisition, the combined tilt, and focal series. This recording scheme combines the advantages of tilt series-based tomography with 3D data acquisition using a focal series and is particularly beneficial for imaging specimens with a thickness of 1 µm or greater.
Dahmke, Indra N. | Hermannsdörfer, Justus | Weatherup, Robert | Hofmann, Stephan | Peckys, Diana B. | de Jonge, Niels
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DOI:de Jonge, Niels | Mølhave, Kristian | Alloyeau, Damien
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DOI:de Jonge, Niels | Peckys, Diana B.
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Electron microscopy of biological cells in liquid provides unique nanoscale information. A highly attractive idea is the capability to also study physiological processes of live cells with electron microscopy. However, this idea seems unrealistic because the minimal needed electron dose to obtain contrast is already many orders of magnitude above the lethal dose known to cause reproductive-cell death. We show here that claims of electron microscopy of viable cells in recent reports are based on a questionable interpretation of the used fluorescence live/dead assay. A practical alternative to study biological processes is correlative light and electron microscopy.
de Jonge, Niels | Ross, Frances M. | Wang, Chongmin
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DOI:Peckys, Diana | Korf, Ulrike | Wiemann, Stefan | de Jonge, Niels
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DOI:Peckys, Diana B. | Alansary, Dalia | Niemeyer, Barbara A. | de Jonge, Niels
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DOI:Peckys, Diana B. | de Jonge, Niels
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DOI:Ross, Frances M. | Wang, Chongmin | de Jonge, Niels
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Transmission electron microscopy is a powerful technique for the analysis of solid samples, but it can also be used to image in liquid environments, gaining a unique view of processes and structures in liquids. Here, we describe recent developments in electron microscopy of liquids and discuss applications in several areas. We first describe closed-liquid-cell microscopy with its opportunities for visualizing electrochemical processes. We then discuss imaging of low-vapor-pressure liquids relevant to the operation of rechargeable batteries. Finally, we describe imaging of thick biological materials to obtain information on membrane proteins in intact mammalian cells that cannot be observed classically under dry or frozen conditions. Electron microscopy in liquid environments is developing rapidly and has the potential to solve key problems in materials science, physics, chemistry, and biology.
Verch, Andreas | de Jonge, Niels
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