{"id":36915,"date":"2025-05-05T08:28:53","date_gmt":"2025-05-05T08:28:53","guid":{"rendered":"https:\/\/www.leibniz-inm.de\/publication\/an-enzymatic-cofactor-regeneration-system-for-the-in-vitro-reduction-of-isolated-cc-bonds-by-geranylgeranyl-reductases\/"},"modified":"2026-05-19T14:43:11","modified_gmt":"2026-05-19T14:43:11","slug":"an-enzymatic-cofactor-regeneration-system-for-the-in-vitro-reduction-of-isolated-cc-bonds-by-geranylgeranyl-reductases","status":"publish","type":"publication","link":"https:\/\/www.leibniz-inm.de\/en\/publication\/an-enzymatic-cofactor-regeneration-system-for-the-in-vitro-reduction-of-isolated-cc-bonds-by-geranylgeranyl-reductases\/","title":{"rendered":"An Enzymatic Cofactor Regeneration System for the in-Vitro Reduction of Isolated C=C Bonds by Geranylgeranyl Reductases"},"content":{"rendered":"

Cofactor regeneration systems are of major importance for the applicability of oxidoreductases in biocatalysis. Previously, geranylgeranyl reductases have been investigated for the enzymatic reduction of isolated C=C bonds. However, an enzymatic cofactor-regeneration system for in\u2005vitro use is lacking. In this work, we report a ferredoxin from the archaea Archaeoglobus fulgidus that regenerates the flavin of the corresponding geranylgeranyl reductase. The proteins were heterologously produced, and the regeneration was coupled to a ferredoxin reductase from Escherichia coli and a glucose dehydrogenase from Bacillus subtilis, thereby enabling the reduction of isolated C=C bonds by purified enzymes. The system was applied in crude, cell-free extracts and gave conversions comparable to those of a previous method using sodium dithionite for cofactor regeneration. Hence, an enzymatic approach to the reduction of isolated C=C bonds can be coupled with common systems for the regeneration of nicotinamide cofactors, thereby opening new perspectives for the application of geranylgeranyl reductases in biocatalysis.<\/p>\n","protected":false},"author":0,"featured_media":0,"menu_order":0,"template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-36915","publication","type-publication","status-publish","hentry"],"acf":[],"yoast_head":"\nAn Enzymatic Cofactor Regeneration System for the in-Vitro Reduction of Isolated C=C Bonds by Geranylgeranyl Reductases - INM<\/title>\n<meta name=\"description\" content=\"An Enzymatic Cofactor Regeneration System for the in-Vitro Reduction of Isolated C=C Bonds by Geranylgeranyl Reductases \u2013 Forschungspublikation des Leibniz-Instituts f\u00fcr Neue Materialien (INM).\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.leibniz-inm.de\/en\/publication\/an-enzymatic-cofactor-regeneration-system-for-the-in-vitro-reduction-of-isolated-cc-bonds-by-geranylgeranyl-reductases\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"An Enzymatic Cofactor Regeneration System for the in-Vitro Reduction of Isolated C=C Bonds by Geranylgeranyl Reductases - INM\" \/>\n<meta property=\"og:description\" content=\"An Enzymatic Cofactor Regeneration System for the in-Vitro Reduction of Isolated C=C Bonds by Geranylgeranyl Reductases \u2013 Forschungspublikation des Leibniz-Instituts f\u00fcr Neue Materialien (INM).\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.leibniz-inm.de\/en\/publication\/an-enzymatic-cofactor-regeneration-system-for-the-in-vitro-reduction-of-isolated-cc-bonds-by-geranylgeranyl-reductases\/\" \/>\n<meta property=\"og:site_name\" content=\"INM\" \/>\n<meta property=\"article:modified_time\" content=\"2026-05-19T14:43:11+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.leibniz-inm.de\/wp-content\/uploads\/2024\/03\/INM-Logo-Regular_en.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"1085\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@Leibniz_INM\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.leibniz-inm.de\\\/en\\\/publication\\\/an-enzymatic-cofactor-regeneration-system-for-the-in-vitro-reduction-of-isolated-cc-bonds-by-geranylgeranyl-reductases\\\/\",\"url\":\"https:\\\/\\\/www.leibniz-inm.de\\\/en\\\/publication\\\/an-enzymatic-cofactor-regeneration-system-for-the-in-vitro-reduction-of-isolated-cc-bonds-by-geranylgeranyl-reductases\\\/\",\"name\":\"An Enzymatic Cofactor Regeneration System for the in-Vitro Reduction of Isolated C=C Bonds by Geranylgeranyl Reductases - 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